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Importance of Sample Preparation and Choosing the Right Gel for SDS-PAGE - Scientific Videos | Thermo Fisher Scientific US

SOLVED: Calculate the preparation of all samples to be run on your SDS-PAGE gel. You need to load 10 μg total protein in 3 μl. You need to prepare 30 μl of this solution that has protein and 1X sample buffer (water is used for dilution). Complete the table below.Sample Protein Protein Water 5X Sample Total Sample Conc. Sample (μg) Buffer Volume (μl) (μl) (μl)CRUDE 30Desalt 30Peak Fraction 30Peak Fraction 30Note: You must complete this table before coming to class.

SOLVED: Investigators decide to analyze the purity of a preparation of antibody molecules using SDS polyacrylamide-gel electrophoresis (SDS-PAGE). In Lane 1 of the gel, they load a sample of the antibody. In Lane 2, they load an antibody sample that has been treated with a reducing agent called mercaptoethanol, which breaks disulfide linkages. Following electrophoresis, they see distinct bands representing polypeptides with molecular weights of 50 kD and 25 kD in Lane 2, and only one band weighi