cDNA Synthesis Protocol by Reverse Transcription
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cDNA Synthesis Protocol by Reverse Transcription
Hey Friends, cDNA (complementary DNA) is synthesized from an RNA template. This is done by Reverse Transcription. In this example, the oligo d(T)-primers are used to prime the cDNA synthesis. An alternative would be to use random hexamers as primers. cDNA is synthesized before qPCR to check gene expression levels. qPCR: https://www.youtube.com ...
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