Real-time PCR offers sensitivity, specificity, and wide dynamic range for detecting target nucleic acids, making it a useful and powerful technology for research applications that cover the spectrum of …

Aug 14, 2025 · Real-Time PCR is a technique used to monitor the progress of a PCR reaction in real-time. At the same time, a relatively small amount of PCR product (DNA, cDNA or RNA) can be …

This guide provides an introduction to many of the technical aspects of real-time PCR. It includes guidelines for designing the best real-time PCR assay for your experiments and explains how real …

Multiple research applications based on qPCR have been developed. These include gene expression analysis, genotyping, allelic discrimination, single nucleotide polymorphism (SNP) detection, microbe …

Sep 21, 2025 · Real-time PCR, also known as quantitative PCR (qPCR), is a molecular biology technique that allows for the amplification, detection, and quantification of DNA or RNA in real-time …

Although various models of real-time PCR are available, all share common features: a standard thermocycler platform coupled with an excitation source (usually a laser or tungsten lamp), a camera …

Quantitative real-time PCR and subsequent amplicon detection is carried out in a closed-tube format which eliminates the need for post-PCR manipulation, such as gel electrophoresis and significantly …

Jul 9, 2025 · Go beyond basic qPCR. Explore advanced real-time PCR applications like KASP and multiplexing to gain deeper insights in gene expression, pathogen detection, and more.

Digital polymerase chain reaction (digital PCR, DigitalPCR, dPCR, or dePCR) is a biotechnological refinement of conventional polymerase chain reaction methods that can be used to directly quantify …

As described on this page, some examples of PCR applications include: 1) gene expression, 2) genotyping (detection), 3) cloning, 4) mutagenesis, 5) methylation analysis, 6) sequencing, and 7) …