A western blot, sometimes called a protein immunoblot, is an antibody-based technique used to detect the presence, size and abundance of specific proteins within a sample. The technique was developed ...

The Western blot workflow for immuno-based protein detection was introduced over 30 years ago. Since its introduction, Western blotting has become a well-known and widely accepted technique routinely ...

This article describes the histone western blot protocol, which is often used at Abcam for identifying histone proteins obtained from purified calf thymus. For each lane, either 0.5 μg of calf thymus ...

Western blotting is a foundational technique for detecting specific proteins in a complex sample. Although blotting protocols are straightforward, many critical decision points can influence the ...

Verify that the antibody is definitely able to detect the antigen in the species by checking data sheets and reference material Potential Cause: Primary and secondary antibody mismatch Raise the ...

Western blots have been a tried-and-true method for separating and quantifying proteins based on molecular weight, but they have not kept up with the continually increasing output demands of modern ...

Western blotting (or immunoblotting) is a well-established biochemical technique that detects specific proteins in complex samples and has applications from basic research to the diagnosis of ...

In this editorial, Kenneth Oh overviews the two main normalization methods used to quantify western blots and discusses common errors to avoid. What is western blot normalization? Kenneth Oh is the ...